Pares Biosciences Single Cell Trascriptomics
Previous generations of single-cell RNA sequencing (scRNA-seq) revolutionized the way researchers explore biology. Recently, Parse Biosciences expanded its adoption with the introduction of Evercode combinatorial barcoding technology. This breakthrough eliminates the necessity for costly equipment, opening doors to exceptional data throughput. Evercode technology excels in detecting genes and cell types with heightened sensitivity, accommodating diverse sample types.
Evercode Whole Transcriptome Kits Offer Following Advantages:
Sequencing Workflow
Step 1: Sample Fixation
Immediately after collecting your samples, ensure the preservation of gene expression with a swift fixation protocol. Following fixation, you have the option to store samples for up to 6 months or proceed directly to the barcoding step.
Step 2: Barcoding and Library Preparation
Enhance each transcript’s traceability by introducing barcodes to individual cells through a split-pool combinatorial barcoding process. Subsequently, the kit advances to standard library preparation, creating sequencing-ready molecules.
Step 3: Sequencing
The libraries produced in the previous step are subjected to Next-Generation Sequencing (NGS).
Step 4: Data Analysis
Our computational pipeline generates an interactive report, providing rapid insights into your data. All resulting data files, such as the gene-cell count matrix, seamlessly integrate with widely-used open-source tools like Seurat or Scanpy for further analysis.
Parse Biosciences Evercode Whole Transcriptome Library Preparation
Applications of Evercode Whole Transcriptome Single-Cell RNA Sequencing:
1010Genome's Evercode Whole Transcriptome scRNA Sequencing Services
At 1010Genome, we understand the intricate language of genes and the importance of deciphering their expressions at the single-cell level. Our Evercode Whole Transcriptome scRNA Sequencing services are designed to provide you with comprehensive insights into the dynamic world of gene expression.
- Unprecedented Sensitivity: We utilize cutting-edge Evercode sequencing technologies to capture the nuances of gene expression at the single-cell level, providing unparalleled sensitivity and accuracy.
- High Throughput: Process thousands of cells simultaneously, allowing for a comprehensive understanding of cellular heterogeneity within a sample.
- Full Transcriptional Coverage: Our services cover the entire transcriptome, ensuring that no crucial information is left undiscovered.
- Single-Cell Resolution: Analyze individual cells to unravel the intricacies of cellular heterogeneity and uncover rare cell populations.
Bioinformatic Analysis Services
Transforming Raw Data into Actionable Insights
Our bioinformatic analysis services are tailored to extract meaningful information from the vast datasets generated by Evercode Whole Transcriptome scRNA Sequencing. We offer a range of analysis options to suit the specific needs of your research:
- Data Preprocessing: Rigorous quality control and preprocessing steps to ensure the accuracy of downstream analyses.
- Clustering and Cell Type Identification: Classify cells based on gene expression patterns, uncovering distinct cell types and subpopulations within your samples.
- Differential Expression Analysis: Identify genes that are significantly upregulated or downregulated across different conditions, shedding light on key biological processes.
- Pathway Analysis: Understand the functional implications of gene expression changes by exploring enriched biological pathways.
- Interactive Data Visualization: Intuitive visualizations that facilitate a deeper understanding of complex biological systems.
Why Choose 1010Genome?
Get Started with Evercode Whole Transcriptomics scRNA Seq Today
Unleash the full potential of your research with 1010Genome's Evercode Whole Transcriptome scRNA Sequencing and Bioinformatic Analysis Services. Contact us to discuss your project, and let us be your trusted partner in unraveling the mysteries of single-cell gene expression.