16s rRNA Data Analysis / Microbial Diversity Analysis

Welcome to 1010Genome, your trusted partner in uncovering the hidden world of microbial diversity through 16S rRNA sequencing analysis. Our state-of-the-art services are designed to empower researchers, healthcare professionals, and organizations in exploring the microbial communities that play a pivotal role in various fields.

What Is 16S rRNA Sequencing?

16S rRNA sequencing is a powerful tool for studying microbial communities. The 16S ribosomal RNA gene is present in all bacteria and archaea and contains regions of both high conservation and variability. By sequencing and analyzing this gene, we can identify and characterize diverse microbial species, allowing us to answer crucial questions in various fields, including: Microbiome Research, Disease Pathogenesis, and Environmental Science.

Heat map 16s rRNA Data Analysis

16s rRNA Importance and Applications

  •  16s rRNA sequencing has been established for the identification and taxonomic classification of bacterial species.
  •  It can prove to be a method for the recognition of novel pathogens.
  •  Being the conserved gene in bacterial species it provides the identification of useful signature sequences and patterns.
  •  16s rRNA sequencing can lead to the development of completely new species.
  •  The conserved variable regions facilitate sequencing and phylogenetic classification.
  •  Researchers can achieve species level sensitivity for metagenomic surveys of bacterial populations.
  •  In clinical microbiology, molecular identification based on 16s rDNA sequencing is applied fundamentally to bacteria whose identification by means of other types of techniques turns out difficult, or requires a lot of time.

16s rRNA Sequencing and Data Analysis

Next-Generation Sequencing Pipelines

16s rRNA Short read libraries target variable V3 and V4 regions of 16s rRNA genes. Although, 16s rRNA sequencing is an amplicon sequencing technique, usually the environment or clinical samples are as clean and need expert hands to process and amplify 16s rRNA genes. Our 16s rRNA sequencing service makes sure that your precious samples are managed in the best possible manner to generate quality sequencing data. Our 16s rRNA sequencing service can help achieve species level sensitivity for biodiversity analysis of bacterial population.

16s rRNA Bioinformatics Analysis Overview

16s rRNA sequencing data analysis requires deep understanding of various steps to be able to generate precise information about biodiversity or phylogeny. A typical data analysis overview for 16s rRNA data analysis pipeline is represented here.

Step-1: Data Quality Control:

The process begins with the examination of raw sequencing data. This step involves assessing data quality, which includes checking for sequencing errors, base call accuracy, and read length distribution. After quality control, the sequences are subjected to preprocessing. This step involves removing low-quality reads, trimming adapters, and merging paired-end reads if applicable.

Step-2: Taxonomic Assignment

The core of 16S rRNA analysis is taxonomic assignment. In this step, sequences are compared to a reference database of 16S rRNA gene sequences. Common databases include Greengenes, SILVA, or the Ribosomal Database Project (RDP). The assignment determines which taxa (such as genera or species) are present in the sample.

Step-3: Operational Taxonomic Unit (OTU) Clustering

The assigned sequences are grouped into Operational Taxonomic Units (OTUs) based on sequence similarity. OTUs are used to define the diversity and composition of microbial communities within the sample.

Step-4: Diversity Analysis

Alpha and beta diversity metrics are calculated to assess the diversity within a sample and the dissimilarity between samples, respectively. This step provides insights into the richness and evenness of microbial communities.

Why Choose 1010Genome for 16S rRNA Sequencing Analysis?

  • Platform Agnostic Approach: We have expertise in working with multiple NGS platforms, including Illumina, Ion Torrent, and PacBio, among others. Our platform-agnostic approach means we can adapt to the sequencing technology used in your project.
  • Customized Pipelines: We have developed customized analysis pipelines that are compatible with the different data formats and outputs of various NGS platforms. This ensures that the analysis is optimized for the specific platform used in your study.
  • Data Integration: Our bioinformatics experts are well-versed in handling data from different platforms and integrating it effectively into the analysis. Whether you've generated short reads, long reads, or a combination of both, we have the tools and expertise to process and interpret the data.
  • Interpretation Across Platforms: Our data analysis is not limited to sequencing technology. We're skilled at interpreting the results, identifying microbial communities, and drawing meaningful conclusions, regardless of the NGS platform.

Key Deliverables for 16s rRNA Data That We Offer

  • Data QC report that includes stats about read trimming and filtering, pair send stitching and chimera filtering.
  • Taxonomy classification report and set of representative sequence file
  • Classified OTU table with abundance information
  • Phylogenetic Tree
  • Alpha Diversity report that includes: Diversity tree, observed species and stats.
  • Beta Diversity report that includes: Trees, Graph and Network diagrams; Distance matrices; PCoA plots

Ready to Explore Microbial Worlds?

Embark on a journey of discovery with 1010Genome's 16S rRNA sequencing analysis services. Whether you're a researcher in academia, a biotech company, or a healthcare institution, our services can uncover the microbial secrets hidden within your samples.