
- Chromatin Immunoprecipitation (ChIP): DNA-protein interactions are stabilized by formaldehyde cross-linking. Cells are then lysed, and chromatin is fragmented into smaller pieces.
- Immunoprecipitation: Specific antibodies are used to selectively pull down the protein of interest along with the associated DNA fragments.
- Library Preparation: The isolated DNA fragments are then subjected to library preparation, where they are tagged and amplified for sequencing.
- Sequencing: The prepared library is sequenced using high-throughput next-generation sequencing platforms, generating millions of short DNA reads.
- Bioinformatics Analysis: Advanced bioinformatics tools are employed to align and map the sequenced reads to the reference genome, revealing the precise locations of protein-DNA interactions.


